Current Issue : April-June Volume : 2012 Issue Number : 2 Articles : 1 Articles
An RP-HPLC method has been developed for the simultaneous estimation of moexipril (MP) and hydrochlorothiazide (HCTZ) in tablet dosage forms. . Optimum separation was achieved by using symmetry C18 (4.6x150 mm, 5, mm) with a mixture of acetonitrile (ACN): Phosphate buffer at pH 3.0 and in the ratio of 43:57 at a flow rate of 0.5ml/min. The detection was carried out at 216nm and retention time was found to be10 min. The developed method was validated as per ICH guidelines. Linearity range was found to be 20 to60 μg/ml forMP&HCTZ. . In the linearity study, regression equation and coefficient of correlation for MP and HCTZ were found to be (y = 269747x +94514.74, r2 =0.999) and (y = 194376x+795863.8, r2 = 0.999) respectively. This newly developed method was successfully utilized for the simultaneous estimation of moexipril andhydrochlorothiazide in pharmaceutical tablet dosage forms....
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